DEVELOPMENT OF SEQUENCE CHARACTERISED AMPLIFIED REGION (SCAR) MARKERS IN <em>ACACIA</em> <em>AURICULIFORMIS</em>

Authors

  • V Yuskianti

Keywords:

Multiplex PCR of SCAR marker, MuPS, genotype identification, DNA marker

Abstract

YUSKIANTI V, XIANG ZB & SHIRAISHI S. 2011. Development of sequence characterised amplified region
(SCAR) markers in Acacia auriculiformis. This paper reports the development of sequence characterised
amplified region (SCAR) markers and their multiplexing for easy, fast and robust identification in Acacia
auriculiformis. A total of 44 of 48 fragments screened using random amplified polymorphic DNA (RAPD)
markers were successfully converted and developed into SCAR markers. Six SCAR markers that had high
discrimination ability and good fragment quality were selected and multiplexed into one set called a multiplex
PCR (polymerase chain reaction) of SCAR (MuPS) marker. Results using 40 individuals of A. auriculiformis
showed that the discriminatory power of each marker ranged between 0.385 and 0.512. However, when
MuPS marker was used the discriminatory power increased to 0.974. When the MuPS was applied to 40 A.
mangium individuals and 16 different Acacia hybrids, the discriminatory power decreased to 0.652 and 0.483
respectively.

Downloads

Download data is not yet available.

Downloads

Published

2022-06-11

How to Cite

V Yuskianti. (2022). DEVELOPMENT OF SEQUENCE CHARACTERISED AMPLIFIED REGION (SCAR) MARKERS IN <em>ACACIA</em> <em>AURICULIFORMIS</em>. Journal of Tropical Forest Science (JTFS), 23(3), 299–304. Retrieved from https://jtfs.frim.gov.my/jtfs/article/view/627

Issue

Section

Articles
Bookmark and Share