MICROPROPAGATION OF ACACIA MANGIUM FROM ASEPTICALLY GERMINATED SEEDLINGS

Abstract

Micropropagation technique for 1-mth-old aseptically germinated Acacia mangium seedlings was developed. Nodal explants from different stern positions of germinated seedlings were segmented to about 2 to 4 mm long and cultured on Murashige and Skoog (MS) basal medium supplemented with 6- benzylamino purine (BAP) and kinetin (Kin) at different concentration levels. MS basal medium with 0.5 mg t¹ BAP was the best combination to induce a higher shoot multiplication with an average of 25.4 shoots per explant. For root formation, excised shoots treated with Seradix 3 (commercial rooting powder) produced 85.0% rooting percentage.