MICROPROPAGATION OF <em>ACACIA</em> <em>TORTILIS</em> SUBSP. <em>RADDIANA</em> AND <em>A. </em> <em>NILOTICA</em> UNDER <em>IN</em> <em>VITRO</em> CONDITIONS

Abstract

Experiments were conducted to investigate propagation of Acacia tortilis subsp. raddiana and A. nilotica in tissue culture. Micropropagation of A.nilotica from nodal segment was achieved on MS medium. The highest average number of shoots per explant (3.58) was obtained on medium containing 2.5 mg I^-1 BA. Individual shoots, when transferred onto half strength MS medium with 4.0 mg I^-1 BA, formed healthy roots in 62.5% of the cultures. Acacia tortilis failed to produce more than one shoot per explant and did not develop roots in any media tested.