EVALUATION OF ANTI-ANGIOGENIC EFFECTS OF 17ΒHNERIIFOLIN DERIVED FROM CERBERA ODOLLAM ON ENDOTHELIAL EA.hy926 CELL LINE.

Abstract

Cerbera odollam (Apocynaceae) plays an important role in the ecological stabilisation of coastal areas. In previous studies, our group discovered that 17βH-neriifolin from this species exhibited in vitro, in vivo and in silico anticancer effects but the anti-angiogenic effect has yet to be investigated. Hence, this study aims to evaluate the in vitro anti-angiogenic effect of 17βH-neriifolin derived from C. odollam and to compare its activity with other well-known cardiac glycosides such as ouabain, digoxin, and digitoxin. Angiogenesis is the formation of new blood vessels from pre-existing ones, plays a crucial role in cancer progression and metastasis. The migration assay was used in this study to evaluate the anti-angiogenic effects of the cardiac glycosides on endothelial cell (EC) EA.hy926. Observation indicated that the percentage inhibition of EC EA.hy926 cell migration ranged from 29.55% to 72.87% for 17βH-neriifolin, 27.96% to 64.72% for ouabain, 30.70% to 64.19% for digoxin, and 35.50% to 63.43% for digitoxin, as compared to the positive control suramin, which ranged from 17.30% to 97.49%. Our results indicate that all four cardiac glycosides exhibited significant anti-angiogenic activities in a dose-dependent manner. This study was conducted entirely in vitro providing valuable insights into the anti-angiogenic potential of these compounds.