INHERITANCE OF ALLOZYME IN SHOREA LEPROSULA (DIPTEROCARPACEAE)

Abstract

Thirty-four to one hundred seeds from 14 half-sib families of Shorea leprosula. were analysed by starch gel electrophoresis in order to verify the genetic control and mode of inheritance of allozyme loci. Of 29 enzyme systems investigated, 11 (AAT, EST, GDH, GPI, IDH, MDH, ME, PGM, SDH, TPI and UGP) exhibited high enzyme activity. To avoid uncertainties in genotyping, enzyme systems from these which were ontogenetically unstable (GDH), tissue specific (AAT), or had complex banding patterns (EST, ME and TPI) were excluded in genetic analyses. Of the remaining 6 enzyme systems (GPI, IDH, MDH, PGM, SDH and UGP), 10 codominant allozyme loci (Gpi-2, Pgm, Idh, Mdh-1, Mdh-2, Mdh-3, Sdh-1, Sdh-2, Ugp-1 and Ugp-2) were intuitively postulated. Genetic analysis utilising single trees and their progenies supported the intuitive interpretation of all 10 proposed loci, with the exception of Mdh-1. For Mdh-3, since maternal heterozygous trees were unavailable, the hypothesis of genetic control must be considered as preliminary.